Hi,
when I set the boot conditions to boot the user program, it doesn't appear to work

1) Schematic failure. Are you sure that your PCB is correct and does provide all signals to MCU right? For example: is EA tied to V_cc?
2) Wrong start conditions. To run user program you may both eject bootloader conditions and set BLJB to correct value (reset checkmark BLJB).
3) Software error. Are you sure that your program has no errors? Have you simulated it in debugger?

Regards,
Oleg