Hi Kai,
Thanks for you reply,
Extremely sorry for delayed response, But I was busy with some unavoidable work and couldn't get time to respond.

1. Power supply plane and ground plane are interrupted at each chip pin row. This results in a loss of solidity of these planes.Try to make these planes run through all the pads, like it's shown here:

The new top and bottom layers posted has power and ground planes running in between pins as well.

2. Do not connect the ground pins of 33pF capacitors to ground plane. They got their ground connection already on top layer. This will reduce ground noise a bit.

My PCB software is not allowing me to remove the conections of 33pF capacitors to GND plane. I will use a gerber editor or Post Script editor to correct above before I give board for making.

3. Power supply plane is relevantly interrupted arround connector X1. If you introduce some bridges for the lines running to this connector, then power supply plane is less broken.

I am sorry I couldnt get exactly what you meant by above. Please explain a bit more.

4. I would insert a soft ferrite bead like BL02RN2 (Murata) or similar where the +5V enters the board, just between power supply connector and the 470µF power supply decoupling capacitor. This will heavily minimize high frequency currents running in the power supply cable!

These beads are not available in local market can you please suggest any other alternative. I am using a line filter for the AC voltage going to the supply transfotmer.

A cable leaves the board arround IC3 and IC4. As you do not provide any common mode filtering, this will result in heavy radiation. The same is valid for the other cables leaving your board.
I would bend each cable arround an individual soft ferrite ring core or feed it through a bigger soft ferrite bead. This will minimize common mode noise on each cable and by this minimize radiation.
In any case, keep the cables as short as ever possible!!

Thanks for the above suggestion but beads are not available here. I will keep the cable length shortest possible. My signal frequency on this cable is very low less than 100Hz do I need ferrites even at this frequency

It can be helpful to insert 10...47Ohm resistors at outputs of 74HC541.

The new layouts contains 10 Ohm resisters at the outputs of 74HC541.

Thank you very much kai for your suggestions.

Regards,
Prahlad Purohit