Hi Abhishek,

you wrote,
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but problem is, we assumed that light source is emitting the spectrum throughout the test and this will not cancel out if intensity changes after blanking and staderization has been done.
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I don't know how is your semi automatic setup. But going by the lab routines your measurment cycle won't take that long for the drift to affect your readings. I have considered the type of result the lab tech will require or expect. Correct me if I am wrong.

If it is allright you can explain your measurement process so that we can have better understanding. If it is of confidential nature and if you wish you can contact me directly.